The Effect of a Static Magnetic Field on microRNA in Relation to the Regulation of the Nrf2 Signaling Pathway in a Fibroblast Cell Line That Had Been Treated with Fluoride Ions

Many physical and chemical factors can influence the Nrf2 signaling pathway, its deregulation has been associated with various disease states. Keap1 molecules are key elements that regulate response under cell stress conditions. Hence, aim of research was to assess impact a static magnetic field generated by permanent magnets on transcriptional activity NFE2L2 KEAP1 genes in human dermal fibroblast line had cultured fluoride ions an environment without them. Additionally, our aimed answer question whether regulatory RNA molecules—miRNAs—are involved these effects. Experiments were performed NHDF treated ions, along simultaneous exposure moderate-intensity field. The RT-qPCR method used determine mRNA level miRNA miR-28-3p, miR-29b-1-5p, miR-141-3p miR-365a-3p. Our results indicate pathway miRNAs regulating it. Moreover, beneficial effect cells fluoride-induced oxidative due stimulating antioxidant defense.